Profile Analysis of Metabolite Fractions from Chamber Bitter Herb (Phyllanthusniruri L.) Ethanol Extract Using Gas Chromatography (GC-MS) Method with Derivatization

Abstract

Analysis of the secondary metabolite profile of chamberbitterherb was conducted by using derivatizationmethod of GC-MS. The purpose of this derivatization is to increase the detection limit and the form of chromatograms, as well as to increase the volatility of non-volatile compounds so the compounds detected in GC-MS are more various. Profiling secondary metabolite using methanol solvents, with RxiTM-1MS capillary columns (30 m x 0.25 mm, outer layer thickness 0.25 ?m). The 70 Ev ionization system was used to detectGC-MS, Helium carrier gas (0.03 Mpa) with a flow rate of 1 mL/min, injector temperature of 250°C, column temperature of 280°C, detector temperature of 110oC and injection volume of 1 µL.The results show that derivatization can increase or improve volatility and stability so it can improve compound detection and improve chromatogram shape. The compounds obtained in the analysis were 31 compounds detected in the ethanol fraction, 25 compounds in the ethyl acetate fraction, and 25 compounds in n-hexane, whereas in previous studies metabolite analysis was carried out without derivatization, the results obtained were 2 compounds detected in the polar fraction, 4 compounds in the semipolar fraction, and 11 components in nonpolar. Compounds obtained included carboxylic acids, amides, amines, phenolic, terpenoids and alcohol.

 Keywords: Secondary metabolite, chamberbitterherb (Phyllanthusniruri L.), GC-MS, derivatization,BSTFA