Rapid Method Analysis for Quantification of Novel Oligosaccharides by Thin Layer Chromatography

Abstract

A novel oligosaccharide containing cashew fruit crush fermentation medium was separated by thin-layer natural action (TLC) technique. The magnitude of the oligo sugar was measured employing a spectrophotometer technique. The results showed that the weight unit of trisaccharide content was comparable to the gram of the samples. The total saccharide content varied among the cashew fruit crush fermentative medium varieties; The SII-6 strains (228 ±0.01mg/ g) contain a lot of oligosaccharide content as compared to SII-16 strains and SI-10 strains.

 Keywords: cashew apple juice, oligosaccharide, raffinose, TLC.