Expression and Purification of Chorismate lyase (UbiC) Extracted from Pseudomonas Putida

Authors

  • Dae-Hyun Kim
  • Myung-Jae Lim
  • Beom Seok Park

Abstract

P-hydroxybenzoic acid (PHBA) is a key component for the production of paraben preservatives and high-performance bioplastics. PHBA is currently produced through the Kolbe-Schmitt reaction based on petrochemicals. However, producing aromatic compounds, such as PHBA, by this reaction is relatively expensive due to the requirement for high temperature and high pressure and problems with byproduct formation. In addition, environmental problems and fluctuations in raw material prices result in a continued decrease in the availability of PHBAs produced by this method. Thus, the production of chemicals using renewable resources, such as microbes, will be a solution to the problems associated with Kolbe-Schmitt reaction-based production of PHBAs.

Chorismate lyase (UbiC) in the Shikimate pathway of Pseudomonas putida KT2440 is produced by converting the intermediate product, chorismite, into PHBA. P. putida is highly resistant to PHBA, and unlike other bacteria, there are few by-products, such as acetate, glycerol or ethanol, formed. We believe that PHBA production through the UbiC mechanism of P. putida is more economical and environmentally-efficient than the current production method. Thus, this study aimed to increase the efficiency of PHBA production by producing and purifying recombinant UbiC as a key enzyme in the production of PHBA by P. putida.

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Published

2019-12-12

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Section

Articles