The Determination of ?-Mangostin Concentration in the Dry Infusion of Mangos teen Pericarp (Garcinia Mangostana L.)

Abstract

use of mangosteen pericarp (Garcinia mangostana L.) as traditional medicine can be manifested in the form of herbal such as infusion. The main compound in mangosteen pericarp is ?-mangostin which supports the inhibition of the growth of cancer cells, antibacterial and antioxidant. This research aims to determine the concentration of ?-mangostin contained in the dry infusion of mangosteen pericarp (Garcinia mangostana L.). This research was carried out by extracting simplicia mangosteen pericarp (Garcinia mangostana L.) using the infundation method. Qualitative analysis of ?-mangostin in dry infusion was carried out by TLC in the stationary phase of silica GF₂₅₄ and the mobile phase of chloroform: ethyl acetate: methanol (80: 10: 5), as well as 10% sulfuric acid spray reagents in ethanol. Quantitative analysis of ?-mangostin in dry infusion was carried out by HPLC in stationary phase C₁₈, mobile phase methanol: formic acid 0.1% in water (75:25) with a flow rate of 1 ml/min at ? 245 nm. TLC chromatograms indicate the suitability of the spots from the samples with the ?-mangostin standard (Rf 0.625). Calculation of ?-mangostin concentration is done with a standard curve ((2.00; 1.50; 1.00; 0.50 and 0.25) x10-2%) with a correlation coefficient of 0.999. The dry infusion of mangosteen pericarp (Garcinia mangostana L.) contains a relatively small concentration of ?-mangostin (0.410 ± 0.039)%.