Optimization and Partial Purification of Protease Enzyme from the Leaves of Citrus Decumana L. (Grapefruit)
Abstract
A protease from Citrus decumana L. leaves was purified by ammonium sulphate precipitation followed by Sephadex G 20 column chromatography. Molecular weight of the enzyme was estimated to be 55kDa by SDS- PAGE. Optimization of pH for protease enzyme was tested using different buffer solutions and maximum activity was observed at pH 6.8 using Tris buffer (0.01M).